GIS – TECHNOLOGY

GIS1: The basic structure of the in vitro system, simulating the gastrointestinal tract, consisted of 1 Duran bottle with a removable screw cap and a 1000 mL capacity made of borosilicate glass. The Duran bottle screw cap had four entries: two for samples and solutions, and the others for the pH meter and temperature sensor. The Duran bottle was put onto a magnetic stirrer with a ceramic heating plate and had a tabulator GL 32 to take the samples.

GIS1 can be used in two phases in the same configuration:

Phase 1: Transit simulation through the stomach and small intestine;
 Phase 2: Transit simulation through the colon in a uni-compartmental system

GIS2: In vitro, colon transit consists of three sections: C1 – ascending, C2 – transverse, C3 – descending colon. Each section consisted of of Duran bottle with a tabulator GL 32 to take the samples. pH was monitored by a digital pH meter. The Duran bottles were put on a magnetic stirrer with a ceramic heating plate. Optionally, another Duran bottle can be added to simulate the transit through the stomach and small intestine.

For example, testing probiotic lactic bacteria strains at the gastric and small intestine level will follow the protocol:

GIS3: In vitro semiautomatic system for testing the viability of microbial strains

The invention consists of an in vitro simulation system of the pig gastro-intestinal tract (stomach and small intestine),in order to determine the viability of probiotic microbial strains. Automation is based on an Arduino Yun module, peristaltic pump and a pH electrode, the simulation being carried out in a Duran GL28 bottle of borosilicate. Automation aims to eliminate the human factor and to simulate in a maximum percent host physiological factors. This represents a strict control of pH at the stomach and small intestine level.
The phases of the simulation in the GIS3 semiautomatic system are:
A Simulation at the stomach level;
B. Simulation at the small intestine level.